Direct probe labeling in a 1NA min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL. Eliminates handling, waste, and regulatory issues associated with the use of radioactivity. No need to strip blots before reprobing. For fast and easy detection of medium to hightarget amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses. Consistent results combining strong signals with very low backgrounds. ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 2NA min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRPcatalyzed breakdown of luminol. Each system includes the following reagents, sufficient for labeling 5 to 1NA µg nucleic acid and detecting 2NANANA to 4NANANA cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.
