During the Western blotting process, nonspecific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham ECL Prime blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents, such as Amersham ECL, ECL start, ECL Prime, ECL Select, and ECL Plex. Each pack contains 40 g of blocking agent, which is sufficient for at least 20 mini blots.
