For the rapid and reproducible isolation of highquality genomic DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells. Highquality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing. Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse. Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and colorcoded kit components Verified in several downstream applications including realtime PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests. The purification process involves minimal shearing resulting in the production of 5 to 1NA μg of good quality, intact genomic DNA from a 2NANA μl sample. Input sample volumes can vary from 5NA μl to 1 ml. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a columnbased, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
