Concanavalin A (Con A) binds molecules that contain αDmannose, αDglucose, and sterically related residues with available C3, C4, or C5 hydroxyl groups Groupspecific adsorbent for molecules containing sugars. Highly suitable for isolation of cell surface glycoproteins from detergentsolubilized membranes Concanavalin A (Con A) binds molecules that contain αDmannose, αDglucose and sterically related residues with available C3, C4, or C5 hydroxyl groups Highly suitable for isolation of cell surface glycoproteins from detergentsolubilized membranes Con A coupled to Sepharose 4B via cyanogen bromide activation. Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing αDmannopyranosyl, αDglucopyranosyl and sterically related residues. The binding sugar requires the presence of C3, C4 and C5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids
