Flexible run protocols – perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples) Flexible throughput – desalt one sample or multiple samples in parallel Good recovery – in the range of 70% to > 95% High desalting capacity – remove > 98% salt with gravity; > 90% with centrifugation Minimal dilution – typically none when used as spin columns; 1.4× when used with gravity flow Time savings compared to dialysis – inexpensive and convenient alternative to lengthy and troublesome dialysis procedures