A protease deficient E. coli host strain for optimal expression of recombinant proteins Highlevel expression of a variety of recombinant proteins Defective in E. coli strain B, and wildtype restriction systems to permit high efficiency transformation with unmodified plasmid DNA Verified by RAPD fingerprint analysis using Ready-To-Go RAPD Analysis Kit Provided in lyophilized form. Well suited for use with the GST Gene Fusion System, E. coli BL21 is an exemplary host for the expression of recombinant proteins (1). As an E. coli B strain, it further lacks the major protease, encoded by the lon gene, catalyzing the endoproteolytic cleavage of damaged and recombinant proteins in the cell (2).
