Epoxyactivated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Epoxyactivated Sepharose 6B is a preactivated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12atom hydrophilic spacer arm Epoxyactivated Sepharose 6B is a preactivated resin for immobilization of various ligands. Epoxyactivated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Other ligands can be coupled through hydroxyl, amino or thiol groups. The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. Epoxyactivated Sepharose 6B is formed by reacting Sepharose 6B with 1,4bis (2,3 epoxypropoxy) butane.
