Fast and simple One step purification of GSTtagged proteins. Mild elution conditions preserving protein antigenicity and function. Easy scaleup by connecting the columns in series. Sepharose 4 Fast Flow provides good flow properties. GST *tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a sitespecific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GSTtagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scaleup.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA™ design or FPLC™ System.
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