Wide fractionation range – Mr 10 000–1 500 000 for globular proteins, well suited for antibody purification High capacity columns – preparative separation of proteins in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively Convenient – prepacked Sephacryl columns are easily connected to ӒKTA systems for automated chromatography at low to medium pressure. Sephacryl S300 HR resin has been used for decades to separate proteins by size exclusion chromatography (SEC; also called gel filtration). Here are a few of the proteins that have been purified using these Sephacryl columns: Intact complement factor H from human plasma. An antibodydendrimer conjugate functionalized with a targeting agent. Soybean mature glycinin A1bB2.