Can be used in combination with a number of column types Includes 2 x 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4) His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidinetagged proteins according to recommended protocols. Moreover, method for optimization of imidazole concentration in the binding buffer is described.
