Simplify small scale purification and expression screening of histidinetagged proteins secreted in eukaryotic cell culture Purify histidinetagged proteins directly from samples that contain metal ionstripping agents and save time and effort Increase target protein yield and decrease degradation through reduced and simplified sample handling Screen histidinetagged proteins in parallel with high reproducibility and reliable results Capture histidinetagged protein from different sample volumes; from low to high milliliter scale Simplify handling using visible and dense Sepharosebased magnetic beads. His Mag Sepharose excel is a magnetic immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions that are strongly bound to a chelating ligand. The medium enables small scale capture, purification, and reproducible screening of histidinetagged proteins expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells. The Mag Sepharose format has excellent properties for small scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidinetagged proteins during the purification procedure. The base matrix is based on Sepharose medium with magnetite incorporated. His Mag Sepharose is available as 1NA% medium slurry in three different pack sizes (2 x 1 ml, 5 x 1 ml, and 1NA x 1 ml) and can be used together with different test tubes and magnetic racks, for example MagRack 6 for test tubes up to 1.5 ml or MagRack Maxi for test tubes up to 5NA ml. 1 ml medium slurry is sufficient for five reactions according to the recommended protocol. The products are delivered in tubes containing 1 ml of 1NA% (v/v) medium slurry that contains 1NANA µl magnetic beads.
