Prepacked with Capto Butyl Excellent for method optimization and parameter screening due to the 1NA cm bed height Easily connected in series to achieve 2NA cm bed height Small bed volume for fast results and minimal sample/buffer consumption Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity HiScreen columns are part of the process development platform available from GE Healthcare. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method
optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production. Capto Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with low to medium hydrophobicity. Capto Butyl often works efficiently with rather low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose 4 Fast Flow. Higher flow rates can be used with Capto Butyl during equilibration, wash and regeneration.
