Phenyl Sepharose 6 Fast Flow is a wellestablished, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.Two levels of substitution help find optimal selectivity and binding capacity for a given application. Prepacked, ready to use columns for convenient process development optimization. 1NA cm bed height of HiScreen columns is designed to allow method optimization and parameter screening. Easily connected in series to achieve 2NA cm bed height. Small bed volume gives fast results and minimal sample/buffer consumption. Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity. The small column volume of 4.7 ml and bed height of 1NA cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scaleup. Process fluid velocities can be applied, since the 1NA cm bed height gives enough residence time and the results can then serve as basis for linear process scaleup. If necessary, two columns can easily be connected in series to give a bed height of 2NA cm. Media prepacked in HiScreen HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
