Optimized for purification of histidinetagged proteins directly from homogenized, unclarified cell lysates. Mild elution conditions preserving protein antigenicity and function. Easy One step purifications of Glutathione STransferase (GST) tagged proteins resulting in high purity. GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA design. HisTrap FF crude is a ready to use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.
