Fast and easy selection with seven different ionexchange ligands on Sepharose Fast Flow and Sepharose XL, prepacked in 1 ml HiTrap columns. Convenient way to screen for the optimal Cytiva anion exchange (aex) or cation exchange (cex) resin for a given application. Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scaleup. HiTrap column format is designed for use with a syringe, peristaltic pump, or chromatography system such as an ÄKTA system. Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly crosslinked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly crosslinked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose XL media have long chains of dextran coupled to a robust, 6% highly crosslinked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethylgroup bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.
