For optimizing purification of histidinetagged proteins when Ni2+ is not the best choice of metal ion. Conveniently charge with your metal of choice. Small bead size for highperformance purification. High binding capacity. IMAC Sepharose HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.
