The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification. Oriented coupling of recombinant Protein A to the matrix via an engineered Cterminal cysteine enhances IgG binding capacity. Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. Prepacked HiTrap columns for process development, screening of purification conditions, and small scale purification of Mabs.
