First choice Protein A resin for routine purification of antibodies: ensures minimized sample dilution and high resolution. Small bead size (34 µm) ensures narrow elution peaks containing concentrated material. HiTrap Protein A HP is packed with Protein A Sepharose High Performance resin. Use this column for routine immunoaffinity purifications of most IgGs from ascites, serum, and cell culture supernatant. These columns deliver highperformance, highresolution purifications with a binding capacity of 20 mg IgG/mL resin. Columns are stable over a wide pH range – operational pH 3 to 9 for use; pH 2 to 10 for cleaning. Successful use in published research Cytiva's HiTrap Protein A HP column is relied upon for a variety of research applications: Researching expression of the CARD15/NOD2 gene in humans, Determining the role of CD8positive cells in radioimmunotherapy in rats, Studying the binding of an IgG conformer to amyloidogenic aggregates Other formats of Protein A Sepharose HP Protein A Sepharose High Performance resin is also available in the following formats: Protein A HP SpinTrap spin columns, for small sample purification using a microcentrifuge, Protein A HP MultiTrap 96well plates, for high throughput screening and small scale purification using centrifuge or vacuum equipment.
