First choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution. The small bead size (34 μm) ensures narrow elution peaks containing concentrated material. High protein recovery due to high binding capacity of 25 mg IgG/mL resin. Broader selectivity compared with protein A ligand. Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system. HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance resin. This protein G affinity resin is a good first choice for generalpurpose capture of antibodies at laboratory scale, because it binds to a broader range of IgG from eukaryotic species and also binds to more subclasses of IgG than protein Abased resins do.
