Hydrolysatefree and animalderived componentfree formulation. Developed for high cell yield and MAb production. ready to use formulation, requires no cholesterol or GS supplementation. Allows for direct or sequential adaptation. Designed for large scale culture applications, including perfusion and fedbatch strategies. Manufactured from traceable components according to cGMP guidelines. Regulatoryfriendly medium designed to increase process yields with a variety of NS0 cell clones. HyClone CDM4NS0 is an animalderived componentfree (ADCF), hydrolysatefree cell culture medium. This regulatoryfriendly medium is developed to increase process yields of a variety of NS0 cell clones in the manufacture of monoclonal antibodies (MAbs). CDM4NS0 medium has been formulated to support the needs of NS0 cell clones and as such does not require cholesterol or glutamine synthetase (GS) supplementation. CDM4NS0 medium has been successfully tested in a variety of culture systems, including Tflasks, shaker flasks, and bioreactors using batch and fedbatch strategies. CDM4NS0 is available in liquid and powder formats in userfriendly packaging.