250× concentrated lipid supplement Animalderived componentfree (ADCF) Developed specifically for NS0 cell cultures Provides cholesterol and fatty acids necessary for optimal NS0 cell culture 250X concentrated supplement for enhanced cholesterol delivery. LS250 lipid cell culture supplements are chemically defined, animalderived componentfree (ADCF). LS250 lipid supplement is specifically developed to stimulate cell growth and monoclonal antibody (MAb) production in cholesterolauxotrophic NS0 cell lines. 250× concentrated lipid supplement formulated using a proprietary lipid complexing process for enhanced stability and filterability. LS250 has been designed to supplement a variety of HyClone serumfree media, and may be used in the preparation of complete liquid media from powdered media (i.e., it may be filtered after dilution in cell culture media). The supplement is designed for hybridoma/myeloma serumfree media (deficient in cholesterol) to meet the cholesterol requirements of auxotrophic NS0 cells.
