Penicillin interferes with the final stage of synthesis of the bacterial cell wall. Streptomycin inhibits elongation at transpeptidation step during protein synthesis and binds to 30S subunit, causing misreading. Common working concentration for penicillin is 100 U/mL; for Streptomycin: 100 μg/mL. Effective against gram negative and gram positive microorganisms. Penicillin/Streptomycin is a broad spectrum bacteriostatic with activity against gram negative and gram positive organisms.
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