HyClone phosphate buffered saline (PBS) cell culture solution includes sodium chloride, sodium phosphate, and potassium without calcium or magnesium. No sodium pyruvate is added. HyClone sodium phosphate buffer is a balanced solution available as 1× and 10× PBS buffer with 0.0067 M PO4, pH 7.0 to 7.2. Balanced salt solution used for multiple cell culture applications. Sterile buffered saline solution: HyClone phosphate buffered saline solutions are filtered through final 0.1 μm poresize filters. Filter integrity tests are performed both before and after filtration. Aseptic fills are performed in a class 100 clean room. Each lot is subjected to sterility testing, which is performed using the membrane filtration method described in the current U.S. Pharmacopeia (USP).