Supplied uncharged, for customized metal ion charging and optimized selectivity. Suitable for optimizing purification of histidinetagged (histagged) proteins when nickel is not the best choice of metal ion. High performance purification due to its 34 μm bead size. High binding capacity. IMAC Sepharose High Performance is supplied free of metal ions, allowing a user to customize the charge to the target protein. Select a transition metal ion (e.g., Cu2+, Zn2+, Ni2+, or Co2+) that will bind to the covalently immobilized chelating ligand on the Sepharose. This resin is commonly used for affinity purification of histidinetagged proteins. However, it is also suitable for purifying native or untagged recombinant proteins.
