Dynamic binding capacity (DBC) higher than 80 g of mAb/L resin at 6 min residence time (RT), saving on resin and buffer consumption. Excellent alkaline stability enables efficient cleaning and sanitization using 0.5 to 1.0 M NaOH for improved process economy and robustness. Wellestablished protein A ligand used with several chromatography matrices. Supported by the PrismA ELISA kit for accurate and convenient detection of leached protein A ligand. Designed for use in short bed height applications using rapid cycling chromatography (RCC) for improved productivity in the protein A capture step. MabSelect PrismA™ X protein A resin is a highcapacity chromatography resin designed to efficiently capture monoclonal antibodies (mAbs). It features exceptional alkaline stability for repeated cleaning in place (CIP) to reduce bioburden. DBC at different residence times The resin is based on a highly crosslinked agarose base matrix with a median particle (bead) size d50v of 50 μm. The DBC for MabSelect PrismA™ X resin using trastuzumab is excellent. At 6 min, the DBC is 82 mg/mL for MabSelect PrismA™ X resin and 74 mg/mL for MabSelect PrismA™ resin. As the high DBC is realized at short RT, the MabSelect PrismA™ X resin is beneficial for use in RCC to further reduce resin volumes to increase grams of mAb produced per liter of resin and decrease overall resin costs. Alkalinestable protein A ligand We developed the ligand used in MabSelect PrismA™ X and MabSelect PrismA™ resins to withstand high concentrations of NaOH. The enhanced alkaline stability lets you perform efficient cleaning of the resin using 0.5 to 1.0 M NaOH over many purification cycles, providing long resin lifetime and minimized risk for bioburden incidents.
