Ni Sepharose excel, 25 mL-CYTIVA 17371201

Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli. Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping. Retained binding capacity of up to 10 mg/mL resin. Simplified sample handling – fewer steps needed compared with conventional workflow. Flexible formats – prepacked and loose resin formats for optimized screening and preparative histag purification. Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.