Reliable and reproducible Clean-Up of proteins and other large biomolecules (5NANANA Mr). Range of applications such as desalting, buffer exchange, and removal of lowmolecular weight compounds. High desalting capacity. Flexible protocols with ability to use gravity flow or centrifugation. Sample preparation in the range of NA.5 to 1.NA ml for gravity and NA.75 to 1.NA ml with centrifugation. PD MidiTrap G25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.NA ml can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple Clean-Up of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation. Instructions supplied with the columns For larger scale please use PD1NA Desalting column (2.5 ml) and for smaller scale, PD MiniTrap G25 (NA.5 ml), PD SpinTrap G25 (13NA µl) and PD MultiTrap G25 (13NA µl in parallel)
