For covalentlylinking DNA fragments in as little as 3NA min. Singledose, ambienttemperaturestable reaction mixes. Catalyzes the formation of a phosphodiester bond between the 5'phosphoryl group and the 3'hydroxyl group of two doublestranded DNA fragments. ATP is required for this reaction. Ligation of bluntended DNA is greatly stimulated by polyethylene glycol. Applications include selfcircularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to bluntended DNA. Each predispensed, singlereaction, ambienttemperaturestable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 2NA µl, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
