For purification of DNA from small molecules by gel filtration. Tested to ensure reproducibly high recovery of DNA. Sephadex G5NA DNA Grade is used in illustra AutoSeq G5NA columns, ProbeQuant G5NA Micro Columns, MicroSpin G5NA Columns, and NICK Columns. Suitable for researchers who prefer to prepare their own columns for purifying nucleic acids. Excellent for rapid purification of newly synthesized oligonucleotides > 10mers, including oligonucleotide probes, in 100 to 150 µL of deprotection solution using spincolumn chromatography Useful for desalting or buffer exchange of DNA and removal of unincorporated radionucleotides from endlabeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL. Sephadex G50 is one of five different Gtypes ranging from G10 for small molecules to G75 for larger molecules. Sephadex G50 is a wellestablished gel filtration resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Alternatively, Sephadex G25 can be used. Sephadex G25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length. Both types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction. MicroSpin columns packed with Sephadex G50 DNA Grade are suitable for the rapid purification of DNA, for use in a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from endlabelled oligonucleotides.
