Manual and automated magnetic isolation of IgA and IgG proteins is accomplished in a single isolation instead of running two separate isolations. Protein A/G binding sites allows use of a broad range of antibody species and subclasses. Low nonspecific binding for fast and clean purification. Fast reaction kinetics increases throughput and precision, and also enables faster movement through viscous solutions. Unique cauliflowerlike surface provides increased area for binding reactions compared to smooth surface particles Uniform, nominal 1 μm diameter provides excellent lot-to-lot reproducibility. SeraMag™ SpeedBeads™ Protein A/G Magnetic Particles provide a fast and convenient method for manual and automated magnetic isolation of affinity bound antibodies or antigens to Protein A/G. These particles can be used for isolating antibodies from serum, cell culture supernatants, or ascites, and for immunoprecipitation and coimmunoprecipitation of antigens from cell or tissue extracts. Isolate IgA and IgG Simultaneously By taking advantage of the affinity provided by both Protein A and Protein G, SeraMag™ SpeedBeads™ Protein A/G Magnetic Particles are a flexible alternative to either Protein A or Protein G alone and can purify IgG from rabbit and mouse serum with higher yield and purity than Protein A and Protein G particles alone. SeraMag SpeedBeads Respond Twice As Fast The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. Two layers of magnetite are coated onto this core, resulting in faster magnetic response times, while the surface is modified with a proprietary method that minimizes nonspecific binding of proteins. Protein A/G is then covalenty bound to the particle surface.
