Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96well filter plates that are ready to use. Fast and flexible protocol with elution conditions formatted for both electrophoresis and LCMS analysis workflows. Useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interaction of 2iminobiotin and streptavidin. Each well is prepacked with Streptavidin Sepharose High Performance for reproducibility and high performance. Easy scaleup to Streptavidin HP SpinTrap or HiTrap Streptavidin HP prepacked columns MultiTrap 96well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems. Streptavidin HP MultiTrap can be used for protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the Streptavidin and the protein of interest is enriched through the affinity interaction with the antibody. Streptavidin HP MultiTrap can also be used for the direct enrichment of proteins that are biotinylated. By binding a specific biotinylated protein to the column, proteinprotein interactions can also be investigated.
