Improves the quality of 2D electrophoresis results by removing interfering contaminants Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics and nucleic acids Delivers quantitative yield in about 9NA min. 2D Clean-Up Kit is designed to prepare samples for 2D electrophoresis that otherwise produce poor 2D spotmaps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution Treatment of the sample with 2D Clean-Up Kit can improve the quality of 2D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2D analysis possible for samples that are otherwise too dirty or too dilute. The 2D Clean-Up Kit procedure uses precipitant and coprecipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for firstdimension IEF The procedure can be completed in 9NA min and does not result in spot gain or loss. The kit contains sufficient reagents to process 5NA samples of up to 1NANA μl each. The procedure can be scaled up for larger volumes or more dilute samples 2D Clean-Up Kit increases labeling efficiency and protein abundance when using 2D Fluorescence Difference Gel Electrophoresis (2D DIGE)
