Contains columns suitable for a successful 2step protocol of human antibodies: HiTrap MabSelect PrismA (1 × 1 mL) and HiPrep 16/60 Sephacryl S300 HR. HiTrap MabSelect PrismA column is prepacked with MabSelect PrismA resin for preparative purification of monoclonal and polyclonal antibodies from serum and cell culture supernatant, providing excellent purity in one step. You obtain final high purity with the HiPrep 16/60 Sephacryl S300 HR size exclusion column, which removes antibody aggregates and/or fragments. Includes instruction with guidance and tips to help you quickly get started. The column package allows you to combine affinity with size exclusion chromatography to achieve very high purity of human IgG. Protein A affinity chromatography captures your antibody The kit includes a HiTrap column prepacked with MabSelect PrismA resin containing a protein Aderived ligand produced in E. coli. The ligand has been specially engineered to create an affinity resin with enhanced alkali and protease stability. The specificity of binding to the Fc region of IgG is similar to conventional Protein A and provides you with excellent purification in one step.
