Contains a HisTrap HP column 1 mL prepacked with Ni Sepharose High Performance (HP) affinity resin for preparative purification of histagged proteins. You achieve final purity with HiPrep 16/60 Sephacryl S200 HR size exclusion chromatography column, which removes any remaining trace of impurities or closely related substances and imidazole. Includes instructions with guidance and tips showing you how to get started. The column package allows you to combine affinity with size exclusion chromatography to obtain highly pure histagged proteins. Nickel ion chelating groups for efficient capture of your histagged proteins Ni Sepharose HP affinity resin consists of highly crosslinked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. Ni Sepharose HP has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery.
