Includes DNA Grade Sephadex G50 rehydrated in doubledistilled water as well as 0.05% Kathon™ CG/ICP Biocide added as a preservative. Preferred loading volume is 15 to 20 µL. The suggested range for loading volumes to achieve optimum purification is 10 to 20 µL. A plate lid may be used during this centrifugation step to improve consistency of volume recovery and reduce the likelihood of contamination during purification steps in sample sequencing. AutoScreen 96Well Plate is used for purification of sequencing samples reactions prior to analysis on capillary array electrophoresis (CAE) sequencers such as MegaBACE 1000 or ABI Prism Sequencing instruments. AutoScreen 96Well Plates used with MegaBACE 1000 can show two to three times lower signal intensities compared with intensities from EthanolPrecipitated reactions. This does not affect read lengths. AutoScreen 96 is also applicable to other size exclusion chromatography applications.
