For the purification of up to 96 PCR products (NA.1 to 1NA kb) simultaneously in as little as 15 min. High yields of pure DNA recovered in a small volume of water or a low ionic strength buffer. Typical recoveries are 85% for PCR products 1NANA bp to 1NA kb in length; salt removal typically ≥ 99%. Prepacked glass fiber matrix 96well plates are validated for use with MachereyNagel NucleoVac 96 vacuum manifold. Avoids ethanol precipitations and hazardous organic extractions. Purified DNA is ready for use in most applications, including fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation. illustra GFX 96 Purification Kit utilizes glass fiber matrix technology in a 96well format for highly efficient purification of PCR products. Fragments from PCR are captured by the matrix in the presence of a chaotropic salt, and contaminants are conveniently removed by washing the matrix with a buffered ethanol solution. illustra GFX 96 PCR Purification Kit contains the following components in sufficient quantities: binding plates, wash plates, collection plates, colorcoded bottles of capture buffer, wash buffer, and a choice of two elution buffers (TrisHCl and sterile water), and an instruction booklet.
