Aliphatic HIC medium designed for intermediate purification and polishing steps Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations BioProcess medium supported for industrial applications The hydrophilic nature of the base matrix ensures low levels of nonspecific binding. Butyl Sepharose High Performance is a member of the GE Healthcare range of HIC media for intermediate purification and polishing of proteins.
Butyl Sepharose High Performance is based on highly crosslinked, 34µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic medium displaying a minimum of ionic interactions.
Butyl Sepharose High Performance media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size. Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient prepacked formats for easy scaleup and process development.
As member of the BioProcess media range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.
