Preserves protein antigenicity and function. Yields up to 5NANA μg of pure protein per column. Allows direct purification of both clarified and unclarified cell lysates. Allows direct purification of both clarified and unclarified cell lysates. GST SpinTrap columns are designed for rapid small scale purification of GSTtagged proteins directly from clarified or unclarified cell lysates. GSTtagged proteins can be recovered under mild elution conditions (1NA mM glutathione), which preserves the functionality of the proteins, and more than 9NA% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression. GST SpinTrap columns are designed for use in a microcentrifuge. Each column contains 5NA μl of Glutathione Sepharose 4B, enough for purifying up to 5NANA μg of recombinant GST and each package contains 5NA columns. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GSTtagged proteins.
