Rapid and convenient small scale purification of IgG. Protein G Sepharose High Performance in prepacked spin columns. No filtration or centrifugation of different crude samples necessary for antibody purification. Classic and crosslink protocols for protein enrichment (immunoprecipitation). Elution conditions formatted for both LCMS and electrophoresis analysis. Elution conditions formatted for both LCMS and electrophoresis analysis. Ab SpinTrap is designed for efficient, small scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included. The spin columns are also suitable for use in antibody screening experiments. Ab SpinTrap columns are used together with a standard microcentrifuge and one purification run is performed in less than 2NA min. Ab SpinTrap contains Protein G Sepharose High Performance, which has high protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media. For optimal performance in antibody purification, use Ab SpinTrap together with buffers prepared using Ab Buffer Kit.
