Each column binds IgY from a onequarter portion of egg yolk (approx. 1NANA mg IgY). For greater capacity, several columns may be connected in series. Packed with a thiophilic adsorption medium, the ligand, 2mercaptopyridine is coupled to Sepharose High Performance. Recovery and purity of IgY are excellent. Can be operated with a liquid chromatography system such as ÄKTA design. The top and bottom frits are manufactured from porous polyethylene Connectors for usage with different chromatography systems and other equipment are included. HiTrap IgY Purification HP is packed with a thiophilic adsorption medium, 2mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by waterstructuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilichydrophobic interaction.
