Suitable for the capture of a wide range of antibody fragments such as antigenbinding fragments (Fabs), domain antibodies (dAbs), and singlechain variable fragments (scFvs). Enables a platform approach to antibody fragment purification. High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin. Convenient for process development, as well as small scale purification. HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system. HiTrap™ Protein L column is packed with Capto™ L resin. This resin combines a rigid, highflow agarose matrix with the immunoglobulinbinding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
