Designed for scaling up purification of histidinetagged proteins High protein binding capacity and minimal leakage of Ni₂₊ ions As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. Available in convenient prepacked HisPrep and HisTrap column formats. Excellent flow properties provide great performance even at high flow rates. Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification. Ni Sepharose 6 Fast Flow is composed of crosslinked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and precharged with Ni₂₊ ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni₂₊ leakage while providing high capacity. Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient prepacked formats for easy scaleup and process development.
As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support
