Highperformance purification of histidinetagged proteins Negligible leakage of Ni2+ ions. High binding capacity, at least 4NA mg/ml medium Compatible with a wide range of reducing agents, detergents, denaturants and other additives 34 µm bead size for improved resolution Ni Sepharose High Performance consists of highly crosslinked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions
