For simple, small scale purification of antibodies or enrichment of specific proteins in prepacked spin columns that are ready to use. Classic and crosslink protocols provide flexibility. Elution conditions formatted for both electrophoresis and LCMS analysis workflows. Fast binding kinetics and high capacity provides high yield. Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses. Easy scale up with HiTrap Protein G HP prepacked columns. Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (crosslink protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 2NA min. No pretreatment of the sample is required.
