Fast, one step purification of MBPtagged proteins. Highresolution purification and elution in narrow peaks, minimizing the need for further concentration steps. Can be used for repeated purification: can be easily regenerated using 0.5 M NaOH. Physiological conditions and mild elution preserve target protein activity. Recombinant proteins are engineered with an MBPtag to facilitate detection, isolation, and purification procedures. In addition, this affinity tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein.
Purification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein. The small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5 M NaOH, allowing the same column to be used to purify different proteins.
