For the isolation and concentration of DNA fragments (5NA bp to 4NA kb) from PCR mixtures, DNA containing agarose gel bands, enzyme based DNA modifications, and restriction enzyme digests. Fast and easy to use method with less than 1NA min handson time. Flexible 1NA to 5NA μl elution volume for different DNA concentration needs. Improved column design with increased maximum volume, holding of up to 8NANA μl liquid volume (95NA μl without a cap). Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning. Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery. Two choices of elution buffer provided to fit downstream applications. Colorcoded caps for ease of use. illustra GFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 5NA bp to 1NA kb. This kit can be used to purify DNA from reaction volumes up to 1NANA μl or agarose gel slices up to 9NANA mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 6NA% to 8NA% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed. illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, colorcoded bottles of capture buffer, wash buffer, and two elution buffers (TrisHCl and sterile water), and an instruction booklet.
