Convenient, prepacked gravity flow columns. Allows direct purification of both clarified and unclarified cell lysates. Yields up to 2NA mg of pure proteins per column. Yields up to 2NA mg of pure proteins per column. GST GraviTrap contains 1NA disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 2NA mg of fusion protein. GSTtagged proteins are recovered under mild elution conditions (1NA mM glutathione), which preserves functionality of the proteins. The binding capacity of prepacked GST GraviTrap columns, which can be greater than 1NA mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GSTtagged proteins.
