For fast and simple purification of histidinetagged* proteins. Convenient, prepacked gravityflow column. Allows direct purification of both clarified and unclarified cell lysates. High binding capacity; approximately 4NA mg of pure protein per column. Compatible with a wide range of reducing agents, detergents, denaturants and other additives. The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickelion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives. His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD1NA Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 1NA ml.
