Convenient histidinetagged purification with high purity ( 9NA%) and binding capacity of up to 15 mg/column. ready to use gravity flow column prepacked with 1 ml TALON® Superflow™ cobaltIMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickelcharged media. One run takes about 3NA minutes, and large sample volumes can be applied all at once. His GraviTrap TALON® gravity flow columns are designed for convenient and efficient gravity flow purification of histidinetagged proteins directly from clarified or unclarified cell lysates. His GraviTrap TALON® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification. Large sample volumes can be applied in one go, and the histidinetagged protein is effectively eluted in a small volume. LabMate PD1NA Buffer Reservoir can be connected to the columns for handling of sample volumes above 1NA ml. Leakage of cobalt ions from TALON® Superflow™ medium is negligible.
